Where Can We Improve Instrument Accuracy?
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use alignment tool to ensure proper angle of cell sectors
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predict accuracy boundary conditions for the cell bottom by measuring rotor stretch
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correct for chromatic aberration in multi-wavlength experiments
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measure in intensity mode and use proper workflow to remove systematic noise
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utilize new timestate structure from Optima for accurate t, omega2t
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accounting for compressibility
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measure at low enough concentration to avoid non-ideality
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optimize signal to noise ratio by selecting proper optical density (different for each wavelength)
Analysis Protocol
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define meniscus and tolerance
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define bottom of cell data limit
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define # of scans and time
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define loading concentration and tolerance
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define sedimentation and frictional ratio fitting limits and grid resolution
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define refinement workflow parameters
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time- and radially-invarianet noise determinations
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meniscus fits
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iterative refinement steps
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etc
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define reference database and reference parameters for hydrodynamic parameters, distribution statistics, RMSD levels, and tolerances.