Where Can We Improve Instrument Accuracy?

  1. use alignment tool to ensure proper angle of cell sectors

  2. predict accuracy boundary conditions for the cell bottom by measuring rotor stretch

  3. correct for chromatic aberration in multi-wavlength experiments

  4. measure in intensity mode and use proper workflow to remove systematic noise

  5. utilize new timestate structure from Optima for accurate t, omega2t

  6. accounting for compressibility

  7. measure at low enough concentration to avoid non-ideality

  8. optimize signal to noise ratio by selecting proper optical density (different for each wavelength)

Analysis Protocol

  1. define meniscus and tolerance

  2. define bottom of cell data limit

  3. define # of scans and time

  4. define loading concentration and tolerance

  5. define sedimentation and frictional ratio fitting limits and grid resolution

  6. define refinement workflow parameters

  7. time- and radially-invarianet noise determinations

  8. meniscus fits

  9. iterative refinement steps

  10. etc

  11. define reference database and reference parameters for hydrodynamic parameters, distribution statistics, RMSD levels, and tolerances.