Planning SV Experiments
Buffer Conditions
Purifying Samples
Buffer Exchange
Establish amounts and concentrations
Self-Association (single pure protein)?
Hetero-association??
Select Rotor Speed
Simulate experiment
Prepare AUC and Cells
Assemble analytical cells
Test run?
Temperature equilibration
Sample Preperation and Start
Prepare mixtures
Fill cells
Seal cell assemblies
Insert rotor
Allow pressure to drop
Prepare for data aquisitoin
Start
Preliminary analysis
Stop and Clean
Recover cells and samples?
Clean cells