Suitable Protein Systems

Protein-protein-interactions can be strong and long-lived or weak and transient.

DOI: 10.1007/s00249-009-0514-1 Brown, Balbo, and Schuck. 2009.

Protein Sci. 2002 Sep; 11(9): 2067�2079. PMCID: PMC2373601PMID: 12192063

PPI are fundamental to function of proteins and life of cell. divided into homo or heterocomplexes presenting as dimers or multimers. strong and long-lived or weak and transient. PPIs can be result of an artefact during preperation and storage of proteins. globular proteins are able to interact due to preforomed surfaces or with an unduced binding surface. protein-peptide interactions, binding is mediated with either a discontinuous or continuous epitope. knowledge of strength and type of interaction is pivotal. analysis allows for understanding role or proteins and protein complexes. strength, stiociometry, dynamics and reversibility can be monitore with auc.

example 1: interaction between HeV V protein and cytoplasmic host proteins drive viral pathogenesis. Atkinson 2018

example 2: trimeric complex FtsQBL controls formation of bacterial divisome. Glas 2015

Analytical ultracentrifugation as a tool for studying protein interactions Harding SE et al. (2010) Biochem. Soc. Trans. 38, 901-907

Analytical ultracentrifugation as a tool for studying protein interactions Schuck P (2013) Biophys Rev 5, 159-171

Recognition by host nuclear transport proteins drives disorder-to-order transition in Hendra virus V Atkinson SC et al. (2018) Scientific Reports 8, 358

The Soluble Periplasmic Domains of Escherichia coli Cell Division Proteins FtsQ/FtsB/FtsL Form a Trimeric Complex with Submicromolar Affinity* Glas M et al. (2015) J Biol Chem 290(35), 21498�21509

Protein-Protein Interactions

(incl. small peptides, glycosylated proteins, membrane proteins, micelles)

Becerra, S.P., Kumar, A., Lewis, M.S., Widen, S.G., Abbots, J., Karawya, E.M., Hughes, S.H., Shiloach, J., and Wilson, S.H. 1991. Protein�protein interactions of HIV-1 reverse transcriptase: Implication of central and C-terminal regions in subunit binding. (Lewis, MS. Appendix: Ultracentrifuge analysis of a mixed association.). Biochemistry 30 11707�11719.

Kar, S.R., Lebowitz, J., Blume, S., Taylor, K.B., and Hall, L.M. 2001. SmtB�DNA and protein�protein interactions in the formation of the cyanobacterial metallothionein repression complex: Zn2+ does not dissociate the protein�DNA complex in vitro. Biochemistry 40 13378�13389

Self Associating Proteins

Bailey, M.F., Davidson, B.E., Minton, A.P., Sawyer, W.H., and Howlett, G.J. 1996. The effect of self-association on the interaction of the Escherichia coli regulatory protein TyrR with DNA. J. Mol. Biol. 263 671�684.

Glycoproteins Shire 1992;

Lewis, M.S. and Junghans, R.P. 2000. Ultracentrifugal analysis of the molecular mass of glycoproteins of unknown or ill-defined carbohydrate composition. Methods Enzymol. 321 136�149.

Membrane Proteins

Reynolds and Tanford 1976

le Maire, M., Champeil, P., and Moller, J.V. 2000. Interaction of membrane proteins and lipids with solubilizing detergents. Biochim. Biophys. Acta 1508 86�111

Heterogeneous Interactions

Protein-Sugar

Ebel, C., Eisenberg, H., and Ghirlando, R. 2000. Probing protein�sugar interactions. Biophys. J. 78 385�393.