Planning SV Experiments

Buffer Conditions

Purifying Samples

Buffer Exchange

Establish amounts and concentrations

Self-Association (single pure protein)?

Hetero-association??

Select Rotor Speed

Simulate experiment

Prepare AUC and Cells

Assemble analytical cells

Test run?

Temperature equilibration

Sample Preperation and Start

Prepare mixtures

Fill cells

Seal cell assemblies

Insert rotor

Allow pressure to drop

Prepare for data aquisitoin

Start

Preliminary analysis

Stop and Clean

Recover cells and samples?

Clean cells