AUC Software Options

DCDT+

DCDT+ is a Windows program for analysis of sedimentation velocity (SV) data and implements fitting of the derived g(s*) distributions as a mixture containing of up to 5 discrete (non-interacting) species.

Developer(s): Walter Stafford; John Philo

System Requirements: Window XP, Vista, 7,8, or 10. Requires Microsoft .NET framework.

Registration: DCDT+ is distibuted as 'freeware' and does not require registration.

Download: link

User Manual: pdf

Stafford, W.F., III. 1992. Boundary analysis in sedimentation transport experiments: A procedure for obtaining sedimentation coefficient distributions using the time derivative of the concentration profile. Analytical Biochemistry 203:295-301.

Philo, J.S. (2000) A method for directly fitting the time derivative of sedimentation velocity data and an alternative algorithm for calculating sedimentation coefficient distribution functions. Analytical Biochemistry, 279, 151-163.

Philo, J. S. (2006). Improved methods for fitting sedimentation coefficient distributions derived by time-derivative techniques. Anal. Biochem. 354, 238-246.

Philo, J. S. (2011). Limiting the sedimentation coefficient range for sedimentation velocity data analysis: Partial boundary modeling and g(s*) approaches revisited. Anal. Biochem. 412, 189-202.

kDalton

The kDalton program directly fits sedimentation equilibrium (SE) scans using non-linear least-squares analysis, providing global analysis of multiple loading concentrations and/or rotor speeds (up to 18 total data sets). It can analyze ideal or non-ideal self-association (with up to 2 different oligomers), and also provides 15 assembly models for mixed associations (hetero-association).

Developer: John Philo

System Requirements: Windows XP, Vista, 7, 8, or 10.

Download: link

Philo, J. S. (2000). Sedimentation equilibrium analysis of mixed associations using numerical constraints to impose mass or signal conservation. Methods Enzymol. 321, 100-120.

Lamm

Discrete species: numerical lamm solution

SedAnal

SedAnal can processs both sedimentation velocity and sedimentation equilibrium data, using the Lamm Equation Filter and Sedimentation Equilibrium Filter, resepctively. SedAnal contains several other programs, such as: DCDT, Multi-Wavlength Analysis (MWL), Wide Distribution Analysis (WDA), and Biospin.

Global analysis of interacting species. Molecular weight anaysis.

Developer: Walter Stafford

System Requirements:

Download: SedAnal link; SedView link

User Manual: pdf link

Stafford, WF, (1998) "Time difference sedimentation velocity analysis of rapidly reversible interacting systems: Determination of equilibrium constants by global non-linear curve fitting procedures." Biophysical Journal,74(2),A301.

Biospin

Roark and Yphantis 1968

Multi-Wavelength Analysis

Walter et al 2015

Wide Distribution Analysis

Stafford, W. F. and E. H. Braswell (2004). "Sedimentation Velocity, Multi-speed Method for Analyzing Polydisperse Solutions." Biophysical Chemistry,108,273-279.

SedFit

c(s). Discret species: approximate Lamm solution. discret species: numerical lamm solution. Realtime isplay and analysis.

For the analysis of sedimentation velocity, dynamic light scattering, and sedimentation equilibrium data. SEDFIT has a variety of models for the analysis with discrete species and size-distributions, including ls-g*(s), c(s), c(M), and c(s, ff0), and their specialized variants.

Developer: Peter Schuck

System Requirements: Windows NT, 95, 98, XP, 2000, Vista, 7, 8

Download: link or NIH link

SedNterp

The SEDNTERP program is a general purpose tool for the interpretation of sedimentation equilibrium, sedimentation velocity, and diffusion experiments.

Developer: John Philo

Download: link

SedPhat

SEDPHAT provides global modeling capabilities for both sedimentation equilibrium and sedimentation velocity data, serving as a platform for the global analysis of a variety of iotherms from different biophysical disciplines.

Developer: Peter Schuck

System Requirements: Windows NT, 95, 98, 2000, XP, or later

Download: link

Svedberg

SVEDBERG is a Windows program for whole-boundary analysis of sedimentation velocity data. It fits directly to the raw scans (usually spanning the entire run) to derive the sedimentation coefficient and molar mass (or, if you prefer, sedimentation coefficient and diffusion coefficient) for up to 6 independent species (mixtures of up to 6 components).

Developer: John Philo

System Requirements: Windows XP, Vista, 7, 8, 10. Requires Microsoft .NET framework.

Registration: SVEDBERG is distributed as 'freeware' and does not require registration.

Download: link

Philo, J. S. (1997). An improved function for fitting sedimentation velocity data for low-molecular-weight solutes. Biophys. J. 72, 435-444.

UltraScan

Main Use: van Holde-Weischet. Discrete species: numerical lamm solution. nonlinear least squares. molecular weight moment analysis. data aquisition. realtime display and analysis. graphics. calculations.

Developer: Borries Demeler

System Requirements:

Registration:

Download: link

link

XL Graph

XLGraph is a general purpose plotting tool for AUC scan data, a utility to transform the raw data plots to help visualize and interpret AUC experiments, and a utility for performing certain mathematical operations on raw AUC scans and then writing out the resultant data as new scan files.

Developer: John Philo

System Requirements: Windows XP, Vista, 7, 8, or 10.

Download: link