AUC Software Options
DCDT+
DCDT+ is a Windows program for analysis of sedimentation velocity (SV) data and implements fitting of the derived g(s*) distributions as a mixture containing of up to 5 discrete (non-interacting) species.
Developer(s): Walter Stafford; John Philo
System Requirements: Window XP, Vista, 7,8, or 10. Requires Microsoft .NET framework.
Registration: DCDT+ is distibuted as 'freeware' and does not require registration.
Download: link
User Manual: pdf
Stafford, W.F., III. 1992. Boundary analysis in sedimentation transport experiments: A procedure for obtaining sedimentation coefficient distributions using the time derivative of the concentration profile. Analytical Biochemistry 203:295-301.
Philo, J.S. (2000) A method for directly fitting the time derivative of sedimentation velocity data and an alternative algorithm for calculating sedimentation coefficient distribution functions. Analytical Biochemistry, 279, 151-163.
Philo, J. S. (2006). Improved methods for fitting sedimentation coefficient distributions derived by time-derivative techniques. Anal. Biochem. 354, 238-246.
Philo, J. S. (2011). Limiting the sedimentation coefficient range for sedimentation velocity data analysis: Partial boundary modeling and g(s*) approaches revisited. Anal. Biochem. 412, 189-202.
kDalton
The kDalton program directly fits sedimentation equilibrium (SE) scans using non-linear least-squares analysis, providing global analysis of multiple loading concentrations and/or rotor speeds (up to 18 total data sets). It can analyze ideal or non-ideal self-association (with up to 2 different oligomers), and also provides 15 assembly models for mixed associations (hetero-association).
Developer: John Philo
System Requirements: Windows XP, Vista, 7, 8, or 10.
Download: link
Philo, J. S. (2000). Sedimentation equilibrium analysis of mixed associations using numerical constraints to impose mass or signal conservation. Methods Enzymol. 321, 100-120.
Lamm
Discrete species: numerical lamm solution
SedAnal
SedAnal can processs both sedimentation velocity and sedimentation equilibrium data, using the Lamm Equation Filter and Sedimentation Equilibrium Filter, resepctively. SedAnal contains several other programs, such as: DCDT, Multi-Wavlength Analysis (MWL), Wide Distribution Analysis (WDA), and Biospin.
Global analysis of interacting species. Molecular weight anaysis.
Developer: Walter Stafford
System Requirements:
Download: SedAnal link; SedView link
User Manual: pdf link
Stafford, WF, (1998) "Time difference sedimentation velocity analysis of rapidly reversible interacting systems: Determination of equilibrium constants by global non-linear curve fitting procedures." Biophysical Journal,74(2),A301.
Biospin
Roark and Yphantis 1968
Multi-Wavelength Analysis
Walter et al 2015
Wide Distribution Analysis
Stafford, W. F. and E. H. Braswell (2004). "Sedimentation Velocity, Multi-speed Method for Analyzing Polydisperse Solutions." Biophysical Chemistry,108,273-279.
SedFit
c(s). Discret species: approximate Lamm solution. discret species: numerical lamm solution. Realtime isplay and analysis.
For the analysis of sedimentation velocity, dynamic light scattering, and sedimentation equilibrium data. SEDFIT has a variety of models for the analysis with discrete species and size-distributions, including ls-g*(s), c(s), c(M), and c(s, ff0), and their specialized variants.
Developer: Peter Schuck
System Requirements: Windows NT, 95, 98, XP, 2000, Vista, 7, 8
SedNterp
The SEDNTERP program is a general purpose tool for the interpretation of sedimentation equilibrium, sedimentation velocity, and diffusion experiments.
Developer: John Philo
Download: link
SedPhat
SEDPHAT provides global modeling capabilities for both sedimentation equilibrium and sedimentation velocity data, serving as a platform for the global analysis of a variety of iotherms from different biophysical disciplines.
Developer: Peter Schuck
System Requirements: Windows NT, 95, 98, 2000, XP, or later
Download: link
Svedberg
SVEDBERG is a Windows program for whole-boundary analysis of sedimentation velocity data. It fits directly to the raw scans (usually spanning the entire run) to derive the sedimentation coefficient and molar mass (or, if you prefer, sedimentation coefficient and diffusion coefficient) for up to 6 independent species (mixtures of up to 6 components).
Developer: John Philo
System Requirements: Windows XP, Vista, 7, 8, 10. Requires Microsoft .NET framework.
Registration: SVEDBERG is distributed as 'freeware' and does not require registration.
Download: link
Philo, J. S. (1997). An improved function for fitting sedimentation velocity data for low-molecular-weight solutes. Biophys. J. 72, 435-444.
UltraScan
Main Use: van Holde-Weischet. Discrete species: numerical lamm solution. nonlinear least squares. molecular weight moment analysis. data aquisition. realtime display and analysis. graphics. calculations.
Developer: Borries Demeler
System Requirements:
Registration:
Download: link
link
XL Graph
XLGraph is a general purpose plotting tool for AUC scan data, a utility to transform the raw data plots to help visualize and interpret AUC experiments, and a utility for performing certain mathematical operations on raw AUC scans and then writing out the resultant data as new scan files.
Developer: John Philo
System Requirements: Windows XP, Vista, 7, 8, or 10.
Download: link